Biochemistry Laboratory (Wet-Lab)

The Biochemistry Lab is a well-equipped facility for sample preparation work. We have all of the equipment needed for the on-site purification of proteins. We ask that any special requirements be discussed with the Scientific Contact well in advance of the experiment to determine feasibility and time requirements.

As a User, you have a safety obligation to label any tubes or containers that you use or generate with a descriptive, but brief, label of the contents of that vessel. Label should include:

  • biological or chemical content
  • concentration
  • date
  • initials
Our freezers and refrigerators are for short-term use only. Please dispose of / ship back to your home institution any tubes, solutions, or samples that you generate while at BioCAT. Those items left in our facility will be purged as part of ongoing maintenance.


If you are seeking use of our Wet-Lab and are not conducting a current experiment as a BioCAT User with scheduled beamtime and a posted ESAF, you must get explicit permission from the Biochemistry Lab Manager, Mark Vukonich, before using the BioCAT Wet-Lab. Also, a "Wet-Lab Only" ESAF must be approved and posted by beamline staff. No equipment or materials in the BioCAT Wet-Lab may be removed, "borrowed," or moved from the BioCAT Wet-Lab.
Any unauthorized personel who are spotted in our Wet-lab, without an approved ESAF, will be in violation of serious safety and security policies at the APS and we will present the matter to the Floor-Coordinators and the heads of Safety and Security at the APS.










BioCAT does provide alot of the basic materials and chemicals that one would require to prepare a sample for a routine setup. However, specialized chemicals, instrumentation, tools, filtering apparatus not found on this page should be supplied by the User.

The easiest means of shipping chemicals to BioCAT is to order them and have them shipped to BioCAT directly. The chemical concentrations found in most buffers and samples will be exempt due to the small quantity found in them. However, all Department of Transportation (DOT) regulations apply and one must take into consideration the hazards and regulations associated with transporting chemicals and samples when shipping anything to BioCAT.

Any chemicals in large quantity or hazardous materials (representing a chemical or biological hazard) must be shipped to BioCAT via building 46 of Argonne National Labs. It is recommended that you work with the shipping coordinator at your institution to ship any of these materials, as the regulations governing the transport of these goods sometimes changes and can be complicated. Very large fines can be accrued, as well, if shipped incorrectly.

It is expected that the user group will furnish their own consumables and will make arrangements for any hazardous waste generated while at BioCAT.

INSTRUMENTATION AND TOOLS


Malvern DLS Nano-S
Cary 50 UV-Vis Spec
BioTek Eon Plate Reader/ microspectrophotometer

Eppindorf 5415R Microfuge
Eppindorf 5415C Microfuge

Sorvall Swing-bucket Centrifuge (15 & 50ml adaptors)
Beckman J-25 Centrifuge w/ JA-20.1 rotor
Beckman TLX Ultra-Centrifuge w/ TLA-100.3 rotor
Beckman Airfuge

Isotemp 210 Water Bath
Isotemp 3006 Circulating Bath

Stemi 2000 and SV6 Stero Microscopes
Nikon Diaphot brightfield/ phase/ epifluorescence
  Inverted Microscope

AKTA FPLC w/ columns
  (check to see if we have a column suitable for your experiment)
4°C, -20°C, -80°C Short-term Storage

Branson Sonifier 450
Sonicator Bath

Denver Instruments Electronic Balances
Micro and Standard pH Electrodes
Pipet-aid Cordless Pipettor
Stirrer/ Hotplates
Glassware
Chemical Fume Hood


CONSUMABLES


Glass and plastic Pipettes
1.5, 5, 15 and 50ml tubes


Deionized water
Acetone
Ethanol

Send mail to vukonich@bio.aps.anl.gov with questions concerning the biochem lab.