Date

BioCAT is offering its eleventh intensive HOW-TO course in BioSAXS. Students will have four days of virtual lectures and hands-on software tutorials on the basics of BioSAXS data collection and processing from expert practitioners in the field. Students will also be able to come on site or mail in samples for data collection on the BioCAT beamline (Sector 18 at the APS) after the lecture portion of the course.

The lectures will take place from 10/7/25 to 10/10/25 and are entirely virtual (via Zoom). Data collection will occur the week of 10/23/25. See the schedule below for details.

Registration

The course will provide a virtual set of lectures and tutorials via Zoom. Participants will be able to ask questions during both lectures and tutorials, and will have time to get help with analysis of their own data. They will also receive downloads of all course materials, including lecture slides and tutorials.

Note: This course is intended for researchers interested in scattering from monodisperse biological macromolecules in solution. It is NOT for soft matter, materials, or systems which are polydisperse.

Participants are also invited to come on-site or mail in samples for data collection and training. Data collection will occur during the week of 10/23/25.

There is no cost associated with the workshop (excepting travel and lodging costs for participants who come on site for data collection, which individual participants must cover) and registration will be granted upon review of a short application.

How to apply

To apply for a spot in the workshop fill out the short application. This requests basic contact and demographic data as well as your experience with SAXS and a paragraph about your research interests and proposed use of SAXS

Applications will be accepted through September 7th 2025, and reviewed the week of September 8th. You will be notified by email about your application.

The application is available here: https://forms.office.com/r/G2qKjt5r2Y

More information

Course topics:

  • Basic principles of SAXS
  • Critical sample preparation and data collection procedures, including online size-exclusion chromatography (SEC-SAXS)
  • Evaluating data quality
  • Basic data processing including size (Rg, Dmax), molecular weight, the P(r) function, and real space reconstructions
  • What you need to know for publication
  • Overview of several advanced and emerging methods
  • Hands-on tutorials with data processing software

Confirmed speakers:

  • Jesse Hopkins (BioCAT, APS)
  • Max Watkins (BioCAT, APS)
  • Michal Hammel (SIBYLS, ALS)
  • Melissa Graewert (EMBL)
  • Thomas Grant (U. Buffalo)
  • Kushol Gupta (U. Pennsylvania)
  • Thomas Weiss (SMB, SSRL)
  • Estella Yee (MacCHESS, CHESS)

If you have questions, please contact Jesse Hopkins (jhopkins1@illinoistech.edu) or Max Watkins (mwatkins2@illinoistech.edu).

Tentative lecture schedule

Note: All times are Central Daylight Time (UTC-5)

Tuesday 10/7/25

10:00 am Welcome
10:05 am SAXS overview
10:35 am SAXS Instrumentation
10:55 am Basic SAXS data validation and analysis
12:10 pm Break
12:45 pm Tutorial: Basic data analysis
03:00 pm End

Wednesday 10/8/25

10:00 am Planning and performing SAXS experiments (inc. complementary methods)
11:15 am SEC-SAXS data collection and analysis
12:00 pm Break
12:30 pm Tutorial: SEC-SAXS data analysis
02:15 pm Q&A Panel
03:00 pm End

Thursday 10/9/25

10:00 am Advanced analysis: Reconstructions and model fitting
10:45 am Publishing your SAXS data
11:00 am Advanced topic: Lipid Nanoparticles (LNPs) studied by SAXS and asymmetric flow field-flow fractionation (AF4/FFF)
11:45 am Break
12:15 am Tutorial: Reconstructions and model fitting
03:00 pm End

Friday 10/10/25

10:00 am Advanced analysis: Introduction to rigid body modeling
10:45 am Advanced analysis: Introduction to flexibility and ensemble modeling
11:30 am Advanced topic: Introduction to FoXS tools and BilboMD
12:15 am Break
12:45 pm Advanced topic: Time resolved SAXS
01:30 pm Planning experiments at BioCAT
01:45 pm Wrap up and final Q&A
02:30 pm End