BioCAT is offering its third MuscleX workshop. The Biophysics Collaborative Access Team (BioCAT) is funded by National Institutes of Health (NIH) with its mission to operate state-of-the-art x-ray facilities for the study of the structure and dynamics of biological systems under non-crystalline conditions similar to their functional states in living tissues. We will have an introductory presentation of the scientific missions supported at BioCAT as well as a series of talks highlighting recent muscle studies either using X-ray diffraction or other structural techniques.
The workshop will take place from 5/18/23 to 5/19/23 and will be entirely virtual (via Zoom). See the schedule below for details.
Registration
All participants this year will be remote participants. There is no cost associated with the workshop, but registration is required for administrative purposes.
How to register
More information
Workshop topics:
- Introduction to scientific mission at BioCAT
- 13 Scientific presentations from recent muscle studies using x-ray
diffraction and/or other structural techniques including the following topics:
- Thick filament-based regulation
- New insights into thin filament regulation
- Titin and muscle regulation
- MyBPC and regulation of thick and thin filaments
- Translational studies on structural bases of muscle diseases
If you have questions, please contact Weikang Ma (wma6@iit.edu).
Confirmed speakers (chronologically):
- Thomas Irving (BioCAT, APS, USA)
- Weikang Ma (BioCAT, APS, USA)
- Yanhong Wang (King’s College London, UK)
- Ilaria Morotti (U Florence, Italy)
- Saffie Mohran (University of Washington, USA)
- Neil Kad (University of Kent, UK)
- Davide Tamborrini (Max Planck Institute of Molecular Physiology, Germany)
- Anthony Hessel (University of Muenster, Germany)
- Caterina Squarci (U Florence, Italy)
- Maicon Landim-Vieira (Florida State University, USA)
- Samantha Harris (U Arizona Tucson, USA)
- Vivek Jani (Johns Hopkins University, USA)
- Farid Moussavi-Harami (University of Washington, USA)
Tentative schedule
Note: All times are Central Daylight Time (UTC-5)
Thursday 5/18/23
| 09:30 am | Welcome and introduction to scientific programs at BioCAT | Thomas Irving |
| . | Thick filament regulation | |
| 10:00 am | Thick filament regulation in porcine myocardium | Weikang Ma |
| 10:40 am | TBD | Yanhong Wang |
| 11:20 am | The effect of inotropic interventions on the regulatory state of the cardiac thick filament | Ilaria Morotti |
| 12:00 pm | Break | |
| 12:30 pm | Biochemically-defined super-relaxed and disordered-relaxed state can be decoupled from the structurally-defined OFF and ON state | Saffie Mohran |
| 01:10 pm | Single-molecule imaging reveals how mavacamten and PKA modulate ATP turnover in myofibrils | Neil Kad |
| 01:50 pm | In situ structures from relaxed cardiac myofibrils reveal the organization of the muscle thick filament | Davide Tamborrini |
| 02:30 pm | Breakout sessions for discussion |
Friday 5/19/23
| . | Titin, Troponin and MyBPC in myofilament regulation | |
| 09:30 am | Titin force in muscle cells alters lattice order, thick and thin filament protein formation | Anthony Hessel |
| 10:10 am | Titin activates myosin filaments in skeletal muscle by switching from an extensible spring to a mechanical rectifier | Caterina Squarci |
| 10:50 am | Cardiac Troponin T N-domain variant destabilizes the actin interface resulting in disturbed myofilament function | Maicon Landim-Vieira |
| 11:30 am | TBD | Samantha Harris |
| 12:10 pm | Break | |
| . | Translational studies in muscle research | |
| 12:40 pm | Right Ventricular Cardiomyocyte Sarcomere Dysfunction and Deficient Thick Filament Activation in Human Heart Failure with Right Ventricular Dysfunction | Vivek Jani |
| 01:20 pm | Myosin activator Danicamtiv increases myosin recruitment and alters the chemomechanical cross bridge cycle in cardiac muscle | Farid Moussavi-Harami |
| 02:00 pm | Myosin in autoinhibited off state(s), stabilized by mavacamten, can be recruited via inotropic effectors | Weikang Ma |
| 02:30 pm | Breakout sessions for discussion |