Date

BioCAT is offering its eighth intensive HOW-TO course in BioSAXS. Students will have four days of virtual lectures and hands-on software tutorials on the basics of BioSAXS data collection and processing from expert practitioners in the field. Students will also be able to mail in samples for data collection on the BioCAT beamline (Sector 18 at the APS) before the course, and there will be time during the workshop to get help with analysis of their own data.

The course will take place from 6/21/22 to 6/24/22 and is entirely virtual (via Zoom). See the schedule below for details.

Registration

All participants this year will be remote participants. The course will provide a virtual set of lectures and tutorials via Zoom, and participants will be able to mail in samples for SAXS data collection at BioCAT the week before the workshop. Participants will be able to ask questions during both lectures and tutorials, and will have time to get help with analysis of the data collected from their mail-in samples. They will also receive downloads of all course materials, including lecture slides and tutorials.

There is no cost associated with the workshop, but initial registration is limited to 15 participants, in order to allow for data collection. A wait list will be kept after registration is filled, and more slots will be opened based on how many of the initial registrants wish to send samples. Registration is on a first-come first-served basis.

How to register

Registration is CLOSED.

Initial registration for the workshop is closed, but you can get on the wait list by contacting Carrie Clark (mclark19@iit.edu). Please include your include your full name, institution, and position (e.g. graduate student, postdoc, professor) in the email.

More information

Course topics:

  • Basic principles of SAXS
  • Critical sample preparation and data collection procedures, including online size-exclusion chromatography (SEC-SAXS)
  • Evaluating data quality
  • Basic data processing including size (Rg, Dmax), molecular weight, the P(r) function, and real space reconstructions
  • What you need to know for publication
  • Overview of several advanced and emerging methods
  • Hands-on tutorials with data processing software

While the primary purpose of the course is educational, participants may mail-in a limited number (1-2) of their own research samples for data collection the week before the course.

While BioCAT supports SEC-SAXS, SEC-MALS-SAXS, and batch mode SAXS, due to time constraints students will be limited to only SEC-SAXS data collection. Please read our guide on how to design a SAXS experiment and how to prepare your SAXS samples.

If you have questions, please contact Jesse Hopkins (jhopkins1@iit.edu).

Confirmed speakers:

  • Jesse Hopkins (BioCAT, APS)
  • Max Watkins (BioCAT, APS)
  • Richard Gillilan (MacCHESS, CHESS)
  • Steve Meisburger (Cornell U.)
  • Tobin Sosnick (U. Chicago)
  • Jeff Wereszczynski Illinois Institute of Technology)

Tentative schedule

Note: All times are Central Daylight Time (UTC-5)

Tuesday 6/21/22

10:00 am Welcome
10:05 am SAXS overview
10:35 am SAXS Instrumentation
10:55 am Planning and performing SAXS experiments (inc. complementary methods)
12:10 am Break
12:40 pm Basic SAXS data validation and analysis
01:55 pm Q&A panel
02:45 pm End

Wednesday 6/22/22

10:00 am Overview of SAXS data processing software
10:15 am Tutorial: Basic data analysis
12:45 pm SEC-SAXS data collection and analysis
01:30 pm Tutorial: SEC-SAXS data analysis
03:00 pm End

Thursday 6/23/22

10:00 am Advanced analysis: Reconstructions and model fitting
10:45 am Publishing your SAXS data
11:00 am Time resolved SAXS
11:30 am Time resolved SAXS application
11:45 am Tutorial: Reconstructions and model fitting
02:15 pm End

Friday 6/24/22

10:00 am Advanced analysis: Introduction to rigid body modeling
10:45 am Advanced analysis: Introduction to flexibility and ensemble modeling
11:30 am Advanced topic: SAXS and intrinsically disordered proteins
12:15 am Break
12:45 pm Advanced topic: SAXS and molecular dynamics
01:30 pm Hands-on: Data analysis of participant’s data
02:30 pm Planning experiments at BioCAT
02:45 pm Wrap up and final Q&A
03:15 pm End