Date

BioCAT is offering its ninth intensive HOW-TO course in BioSAXS. Students will have four days of virtual lectures and hands-on software tutorials on the basics of BioSAXS data collection and processing from expert practitioners in the field. Students will also be able to mail in samples for data collection on the BioCAT beamline (Sector 18 at the APS) before the course, and there will be time during the workshop to get help with analysis of their own data.

The course will take place from 2/28/23 to 3/3/23 and is entirely virtual (via Zoom). See the schedule below for details.

Registration

All participants this year will be remote participants. The course will provide a virtual set of lectures and tutorials via Zoom, and participants will be able to mail in samples for SAXS data collection at BioCAT the week before the workshop. Participants will be able to ask questions during both lectures and tutorials, and will have time to get help with analysis of the data collected from their mail-in samples. They will also receive downloads of all course materials, including lecture slides and tutorials.

Note: This course is intended for researchers interested in scattering from monodisperse biological macromoleculesin solution. It is NOT for soft matter, materials, or systems which are polydisperse.

Registration will be limited to 30 participants in order to allow for data collection and one-on-one and/or small-group interactions between participants and instructors. We will offer 10 participants the opportunity to supply samples for the workshop. These participants will mail in samples for data collection on the BioCAT beamline (Sector 18 at the Advanced Photon Source) before the course. There is no cost associated with the workshop and registration is on a first-come first-served basis.

How to register

Registration is CLOSED.

More information

Course topics:

  • Basic principles of SAXS
  • Critical sample preparation and data collection procedures, including online size-exclusion chromatography (SEC-SAXS)
  • Evaluating data quality
  • Basic data processing including size (Rg, Dmax), molecular weight, the P(r) function, and real space reconstructions
  • What you need to know for publication
  • Overview of several advanced and emerging methods
  • Hands-on tutorials with data processing software

While the primary purpose of the course is educational, participants may mail-in a limited number (1-2) of their own research samples for data collection the week before the course.

While BioCAT supports SEC-SAXS, SEC-MALS-SAXS, and batch mode SAXS, due to time constraints students will be limited to only SEC-SAXS data collection. Please read our guide on how to design a SAXS experiment and how to prepare your SAXS samples.

If you have questions, please contact Jesse Hopkins (jhopkins1@iit.edu).

Confirmed speakers:

  • Jesse Hopkins (BioCAT, APS)
  • Max Watkins (BioCAT, APS)
  • Richard Gillilan (MacCHESS, CHESS)
  • Thomas Grant (U. Buffalo)
  • Kushol Gupta (U. Pennsylvania)
  • Steve Meisburger (Cornell U.)
  • Tanja Mittag (St. Jude)
  • Susana Teixeira (U. Delaware/NIST Center for Neutron Research)

Tentative schedule

Note: All times are Central Standard Time (UTC-6)

Tuesday 2/28/23

10:00 am Welcome
10:05 am SAXS overview
10:35 am SAXS Instrumentation
10:55 am Planning and performing SAXS experiments (inc. complementary methods)
12:10 am Break
12:40 pm Basic SAXS data validation and analysis
01:55 pm Q&A panel
02:45 pm End

Wednesday 3/1/23

10:00 am Overview of SAXS data processing software
10:15 am Tutorial: Basic data analysis
12:45 pm SEC-SAXS data collection and analysis
01:30 pm Tutorial: SEC-SAXS data analysis
03:00 pm End

Thursday 3/2/23

10:00 am Advanced analysis: Reconstructions and model fitting
10:45 am Publishing your SAXS data
11:00 am Advanced topic: SAXS and SANS
11:45 am Tutorial: Reconstructions and model fitting
02:15 pm End

Friday 3/3/23

10:00 am Advanced analysis: Introduction to flexibility and ensemble modeling
10:45 am Advanced analysis: Introduction to rigid body modeling
11:30 am Advanced topic: SAXS used to study liquid-liquid phase separation (LLPS)
12:15 am Break
12:45 pm Advanced topic: Time resolved SAXS
01:30 pm Hands-on: Data analysis of participant’s data
02:30 pm Planning experiments at BioCAT
02:45 pm Wrap up and final Q&A
03:15 pm End