BioCAT at Biophysics 2020 in San Diego

The BioCAT booth at the 2020 biophysical society meeting.

Please come and visit us at booth number 430 at the Annual Meeting of the Biophysical Society in San Diego February 15-19 for swag and good conversation.

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Everything BioSAXS 5 Workshop Wrap-Up

BioCAT held its fifth intensive HOW-TO course in BioSAXS from 11/5/19-11/7/19 with 13 on-site participants and 24 remote participants. There was a day and a half of lectures and hands-on software tutorials on the basics of BioSAXS data collection and processing from expert practitioners in the field. This was followed by data collection on the BioCAT beamline and data analysis help for on-site participants. Many students brought research samples for the data collection portion of the workshop.
Participants and instructors at the Everything BioSAXS 5 Workshop.

BioCAT held it’s fifth annual BioSAXS training course from 11/5/19-11/7/19. There were 13 on-site participants, 24 remote participants, and 6 instructors. Day one started off with an excellent overview of the basic physics of SAXS and what kind of information you can obtain from the technique by Dr. Xiaobing Zuo (12ID, APS). This was followed by a talk from Dr. Kushol Gupta (Penn. State) going over the extremely important and sometimes overlooked steps of how to actually plan and perform SAXS experiments. He covered everything from planning what data you want to collect to sample preparation for the experiment, to complementary biophysical techniques for …

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Register for Everything BioSAXS 5: Getting started in biological small-angle x-ray solution scattering

BioCAT is offering its fifth intensive HOW-TO course in BioSAXS. Students will have two days of lectures and hands-on software tutorials on the basics of BioSAXS data collection and processing from expert practitioners in the field. This will be followed by data collection on the BioCAT beamline (Sector 18 at the APS) and data analysis help. Students are encouraged to bring 1-2 research samples for the data collection.

The course will take place from 11/5/19-11/7/19 at the APS (see schedule below for details).

For the first time ever, BioCAT is offering this course for remote participants. Remote participants will get a live-streamed feed of the lectures and all of the tutorial materials. They will not be able to collect data.

Participants and instructors at the BioCAT Everything BioSAXS 4 course in 2018.

Registration types and cost

BioCAT is offering two methods of participation for this workshop. The first is on-site, the second is remote.

On-site

The on-site participant registration includes the lectures, hands-on training, and data collection. Lunch and coffee breaks are provided. Due to time constraints on data collection we can only accept 15 on-site participants.

On-site cost: $150/$300 for academic/industrial

Remote …

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Everything BioSAXS 4 Workshop Wrap-Up

BioCAT held its fourth intensive HOW-TO course in BioSAXS from 11/5/18-11/7/18. The 11 participants received a day and a half of lectures and hands-on software tutorials on the basics of BioSAXS data collection and processing from expert practitioners in the field. This was followed by data collection on the BioCAT beamline and data analysis help. Many students brought research samples for the data collection portion of the workshop.
Participants and instructors at the Everything BioSAXS 4 Workshop.

BioCAT held it’s fourth annual BioSAXS training course from 11/5/18-11/7/18. There were 11 participants and 6 instructors. Day one started off with breakfast, followed by an excellent overview of the basic physics of SAXS and what kind of information you can obtain from the technique by Dr. Xiaobing Zuo (12ID, APS). This was followed by two talks from Dr. Lokesh Gakhar (Iowa State) going over the extremely important and sometimes overlooked steps of how to actually plan and perform SAXS experiments. He covered everything from planning what data you want to collect to sample preparation for the experiment, to recommended notes you should take while at the beamline. Following that Dr. Srinivas Chakravarthy …

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SAS2018: BioSAS Advanced Analysis Workshop Wrap-Up

Dr. Jesse Hopkins of BioCAT was a co-organizer of the BioSAS Advanced Analysis Workshop workshop at the XVII International Small Angle Scattering Conference (SAS2018). The workshop focused on advanced techniques for analyzing biological scattering data from three different software packages: BioXTAS RAW, US-SOMO, and SASSIE-web. Twenty three participants from around the world joined six expert instructors for the one-day workshop on October 6th 2018.
Participants and instructors at the SAS2018 BioSAS Advanced Analysis Workshop.

Solution small-angle x-ray and neutron scattering methods are having an increasing impact in structural biology largely due to advanced hardware, data analysis and modeling. The objective of this workshop was to give users hands-on practical experience with a variety of software tools that provide powerful capabilities for processing, analyzing and modeling BioSAS experimental data. This workshop introduced participants to advanced analysis techniques including deconvolving SEC-SAS data sets and utilizing molecular dynamics in combination with SAS curve fitting. The software used was the BioXTAS RAW, SASSIE-web and US-SOMO software suites. The instructors were Emre Brookes (UTHSCSA), Joseph Curtis (NIST), Jesse Hopkins (BioCAT, APS), Richard Gillilan (MacCHESS), Susan Krueger (NIST), Mattia Rocco (HSanMartino, Italy) and other available staff. Participants were given a mixture …

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Register for Everything BioSAXS 4: Getting started in biological small-angle x-ray solution scattering

BioCAT is offering its fourth intensive HOW-TO course in BioSAXS. Students will have a day and a half of lectures and hands-on software tutorials on the basics of BioSAXS data collection and processing from expert practitioners in the field. This will be followed by data collection on the BioCAT beamline (Sector 18 at the APS) and data analysis help. Students are encouraged to bring 1-2 research samples for the data collection.

The course will take place from 11/5/18-11/7/18 at the APS (see schedule below for details).

Students at the BioCAT BioSAXS course in 2016.

How to register

Registration is CLOSED.

Registration is first come, first served, and limited to the first 15 participants due to time constraints on how many users can collect data during the course.

Cost

$200/$300 for academic/industrial

Lodging and Travel

Participants are responsible for their own lodging and travel. We recommend participants plan to arrive Sunday (11/4) and leave Thursday morning (11/8). Rooms are available at the Argonne Guest House.

More information

While the primary purpose of the course is educational, students may bring a limited number (1-2) of their own research samples. Prepared standard protein samples …

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MuscleX: Muscle X-ray Diffraction Workshop Wrap-Up

The MuscleX workshop was a great success with 63 registered attendees!

BioCAT Workshop Itinerary

Madison, Wisconsin 5/23/2018

1:00 PM BioCAT Update and Overview - Tom Irving (BioCAT)

1:30 PM New opportunities with intact and skinned skeletal muscle - Weikang Ma (BioCAT)

2:00 PM Best Practices for X-ray Diffraction Experiments with Cardiac Muscle - Vickie Yuan (U Washington)

2:30 PM Multiscale modeling, X-ray patterns, and estimation of forces on myofilaments in intact muscle - Srba Mijailovich (IIT)

3:00 PM Break

3:20 PM New opportunities for SAXS and muscle proteins - Jesse Hopkins (BioCAT)

3:40 PM MuscleX: New tools for analysis of X-ray diffraction patterns - Weikang Ma (BioCAT)

4:00 PM Structured Discussion: Strategic Directions for the Muscle Diffraction Program at BioCAT - Massimo Reconditi (U. Florence) and Pieter de Tombe (Imperial College, London)

5:00 PM Adjourn

5:00 - 6:00 no host cash bar reception

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MuscleX: Muscle X-ray Diffraction Workshop

We are pleased to announce the first BioCAT Muscle X-ray Diffraction Workshop on Wednesday 5/23/2018, from 1 to 5 pm in the Founder’s Room of the Hilton Madison Monona Terrace Hotel, 9 East Wilson Street, Madison, WI 53703, USA.

This is immediately after the conclusion of the 6th Biennial Myofilament Conference, “Elastic domains in proteins of the sarcomere: Stressors, regulators or rulers?”

There will be no additional charge for attendees of the workshop.

Preliminary program details will be available soon, please check back for updates!

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BioCAT user Joshua Riback wins 2018 Rosalind Franklin Investigator Award

A University of Chicago graduate student in Biophysical Sciences has received the 2018 Rosalind Franklin Investigator Award from the Advanced Photon Source (APS) Users Organization at the U.S. Department of Energy’s (DOE) Argonne National Laboratory.

Joshua Riback, who is in his fifth year of graduate studies, was recognized for his work using small-angle X-ray scattering (SAXS) techniques at the APS to study biophysical interactions.

Riback’s research focuses on the link between the biophysical properties of macromolecules and the principles of biological phenomena, such as subcellular localization and compartmentalization, evolution and fitness. By focusing on how proteins respond when temperature increases, Riback is working toward understanding the breadth of mechanisms that occur in the temperature-dependence and specificity of protein assembly.

“I was quite amazed at the work being done at the synchrotron, especially how many different techniques by many different fields seem to be taking part at the same time.” — Joshua Riback, University of Chicago graduate student and APS user

Ultimately, the goal is to extrapolate these mechanisms to other biological signals or stresses, so as to develop our understanding of the physical basis and cellular benefits of assembly.

Riback first worked on a light source during his …

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